How to Set Up an ELISA

The enzyme-linked immunosorbent assay (ELISA) represents a simple and sensitive technique for specific quantitative detection of molecules to which an antibody is available (1,2 ). Although there are a huge number of variations based on the original ELISA principle, this chapter will focus on the two perhaps most useful and routinely performed: the indirect sandwich ELISA, providing high sensitivity and specificity; and the basic direct ELISA, useful when only one antibody to the sample antigen is available.