Production and Characterization of Humanized Rag2/c/ Mice

Mice reconstituted with human immune cells represent a model to study the development and functionality of the human immune system. Recent improvements in humanized mice have resulted in multi-lineage hematopoiesis, prolonged human cell engraftment that is detectable in many mouse organs, and the ability to generate de novo human innate and adaptive immune responses. Here, we describe the methods used to produce and characterize humanized Rag2^−/− γc^−/− mice.