Contiguation of Bacterial Clones

For accurate assembly of a bacterial clone map (or contig), it is necessary to precisely detect the degree of overlap and map order of clones. One way is to generate fingerprints for all the clones in a given project. The overlap between clones is determined by the proportion of comigrating restriction fragments on either acrylamide or agarose gels, with mulitfold coverage of clones allowing rapid identification of artifacts such as rearranged or deleted clones.