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Inhibition of the SUMO Pathway by Gam1

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We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro . Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.
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