FA Extraction of [insoluble] Amyloid beta from Mouse Brain
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实验试剂
(1M Tris base, 0.5 M Na2 HPO4 , 0.05% NaN3 )
4. H2 O to 500 ml; pH is not adjusted; store and use at room temperature.
5. CAUTION: Sodium azide (NaN3 ) is highly toxic.
实验步骤
3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor).
4. Dilute 210 μl supernatant into 4 ml of room temp FA Neutralization Solution. Mix briefly.
6. Aliquot and flash freeze on dry ice.
注意事项