Lysis Buffers提取蛋白的细胞裂解溶液【UCSF】

TGEK Base

50 mM Tris

10% vol Glycerol

1 mM EDTA

100 mM KCl

(add for std lysis buffer):

PMSF

Benzamidine

Leupeptin

Aprotinin

0.5% NP40

(add for solubilization buffer)

1% NP40

0.1% Triton X100

0.1% SDS

0.5% NaDeoxycholate

Buffer A (fractionation buffer)

10 mM Tris

1.5 mM MgCl2

10 mM KCl

Buffer C (nuclear lysis buffer)

25% glycerol

1.5 mM MgCl2

0.2 M EDTA

0.42 M NaCl

10 mM Tris

RIPA

50mM Tris HCl

150 mM NaCl

1% NP40

0.5% NaDeoxycholate

0.1% SDS

Ubi Buffer 1

(base)

50 mM Tris pH 7.4

1 mM EDTA

250 mM NaCl

0.1% NP40

1% Triton X100

0.5% SDS

0.25% DOC

(add)

100 mM NaF

5 mM NaVO3

Pepstatin

PMSF

Aprotinin

Benzamidine

Leupeptin

Ubi Buffer 2

(base)

50 mM Tris pH 7.4

1 mM EDTA

0.1% NP40

250 mM NaCl

(add)

5 mM NaF

0.5 mM NaVO3

Pepstatin

PMSF

Aprotinin

Benzamidine

Leupeptin

NP40

20 mM Tris pH 7.5

10 mM B-Glycerophosphate

2 mM EGTA

5 mM NaF

150 mM NaCl

1 mM NaPPi

1% NP40

(add)

2 mM DTT

1 mM Vanadate

.5% Aprotinin

100 uM PMSF

(or for IP,add)

Aprotinin

Pepstatin

Benzamidine

Leupeptin

PMSF

5mM Vanadate

5mM DTT