Markers, Selection, and Media in YAC Cloning

The genetic markers used in yeast, and in yeast artificial chromosome (YAC) cloning, are largely defined by the manipulation of growth media. This chapter, apart from providing recipes for media formulation, also contains a brief introduction to genetic markers in yeast that are relevant to YAC cloning. It is hoped that this will provide an adequate understanding of the media that are used for particular applications, and allow adaptation of media for individual requirements.