激光显微切割 Laser Microdissection protocols

激光显微切割 Laser Microdissection protocols

Discussion of Laser Microdissection Projects
Since ideal tissue preparation parameters for mesenchymal tissues and epithelial tissues differ and because considerations of fixation, protocols may vary depending upon the ultimate purpose of the microdissected samples. Discussion of your project in advance is recommended.

Identifying Areas To Microdissect
You should be aware that identification of structure in uncoverslipped slides is not as easy as on coverslipped preparations. Double-scoping potential samples on the morphology reference (Map) slide to identify areas for microdissection with a pathologist is recommended. Scheduled time with a pathologist can be arranged.

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Current LM Protocols

• Optimal Tissue Collection
• Frozen Section Preparation
• Frozen Tissue Storage
• Frozen Section Staining
• Example of Different Staining Protocols
• Paraffin Block Preparation
• Paraffin Slide Preparation
• Paraffin Section Staining

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