Quantification of PCR using delta-delta Ct method.-Molec
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To me it seems that the "Ct-value" is a exponetial value. Is this right? Does anyone have any comment on this?
-neven-
Of course the values are exponential as the PCR reactions are exponential functions..
In the delta-delta ct method usually the expression is normalized against a housekeeping gene, the given difference (delta-ct) is then substracted from the calibrator sample resulting in the delta-delta-ct value.
-Charon-
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