Annexin V detection protocol
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实验原理
实验步骤
Example protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085):
1. Incubation of cells with Annexin V-FITC
1) Induce apoptosis by desired method.
2) Collect 1-5 x 105 cells by centrifugation.
3) Resuspend cells in 500 μl of 1X Binding Buffer.
4) Add 5 μl of Annexin V-FITC and 5 μl of propidium iodide (PI, optional.)
5) Incubate at room temperature for 5 min in the dark.
6) Proceed to B or C below depending on method of analysis.
2. Quantification by flow cytometry
