ACETYL CHOLINESTERASE

 

SECTIONS

Unfixed cryostat or cold Formol calcium/gum sucrose fixed sections.

 

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SOLUTIONS

a. Incubating solution.

--acetyl thiocholine iodide 5mg

--0.1M acetate buffer (pH.6.0) 6.5ml

--0.1m sodium citrate (2.94g/100ml) 0.5ml

--30mM copper sulphate (0.58g/100ml) 1.0ml

--Distilled water 1.0ml

--5mM potassium ferricyanide (0.165g/100ml) 1.0ml

--Add in order, mixing well at each stage

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METHOD

1. Incubate in solution a. at 37� for 15-120 minutes.

2. Rinse in distilled water.

3. Counterstain in Haematoxylin.

4. Dehydrate, clear . Mount sections in DPX

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RESULTS

Cholinesterase activity Red/brown.

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NOTES

i) Care should be taken when using cholinesterase inhibitors as they are extremely toxic.

ii) Unfixed cryostat sections of rectal biopsy take one hour.

iii) Unfixed cryostat sections of muscle biopsy take one and half to two hours.