Cryopreservation of Fish Spermatozoa

Cryopreservation of fish spermatozoa has closely shadowed historical developments of the preservation technology used for mammalian gametes. Within 4 yr of the discovery of the cryoprotective value of glycerol (1 ), slices of Atlantic herring (Clupea harengus ) testis were successfully cryopreserved and stored in dry ice for 6 mo in 80% (v/v) seawater containing 12.5% (v/v) glycerol (2 ).