Cryopreservation of Fish Sperm
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This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles described
can be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fish
spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration conditions, choice
of cryoprotectant medium, cooling/thawing regimes, and conditions for fertilization. Even though some general rules can be
applied to any fish species, optimization of the protocol is needed for each individual species. Furthermore, because sperm
derived from marine and freshwater fish differs markedly, the described protocol is mainly focused on the cryopreservation
of sperm for freshwater fish because this poses the greater technical challenge.