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Assay of Protein Kinases and Protein Phosphorylation

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Part A. Protein Kinase-Mediated Phosphorylation Events
Protein kinases (ATP:protein phosphotransferases) regulate a wide range of cellular events, including the transduction of signals leading to cell growth. Although the protein kinase superfamily encompasses a large and structurally diverse group of enzymes, protein and DNA sequencing data indicate that they share a common evolutionary origin with a particularly highly conserved catalytic core structure and function. At present, the well characterized protein kinases (PK) can be divided into four main subclasses
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