Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay
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1. | Radioimmunoassay (RIA), using a high specific activity adenosine 3′,5′-cyclic phosphoric acid 2′-0 -succinyl-3-[125 I]iodotyrosine methyl ester together with a second antibody that is bound to magnetizable polymer particles. |
2. | Enzyme immunoassay (EIA), involving the linking of succinyl cAMP to horseradish peroxidase (HRP) and combining this with stable second-antrbody-coated microtiter plates. |
3. | Homogeneous RIA (nonseparation), incorporating the novel method of scintillation proximity assay (SPA). |