海洋微生物的培养基:Marine Agar with Sulfur(ATCC Medium 1922)
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Composition per liter:
NaCl ……19.45g
Sulfur ……10.0g
MgCl2……8.8g
Peptone……5.0g
Na2SO3……3.24g
CaCl2……1.8g
Yeast extract ……1.0g
KCl……0.55g
NaHCO3 ……0.16g
Ferric citrate……0.1g
KBr……0.08g
SrCl2……0.03g
H3BO3 ……0.02g
Na2HPO4 ……8.0mg
Na2SiO3……4.0mg
NaF……2.4mg
NH4NO3 ……1.6mg
pH 7.6 ± 0.2 at 25°C
Preparation of Sulfur: Autoclave sulfur for 15 min at 0 psi pressure–100°C on three successive days.
Preparation of Medium: Prepare anaerobically under a gas phase of 80% N2 + 10% CO2 + 10% H2.
Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50–55°C. Aseptically add 10.0g of sterile sulfur.
Mix thoroughly. Aseptically and anaerobically, under a gas phase of 80% N2 + 10% CO2 + 10% H2, distribute into sterile tubes.
Use: For the cultivation and maintenance of Thermococcus litoralis.