Site-Specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes
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Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.
![2-[carboxymethyl-[(1R,2R,3R,5S)-2,6,6-trimethylnorpinan-3-yl]amino]acetic acid;2,2,2-trifluoroacetic acid，2940871-76-9，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/744/2918217110184523081.jpg!wh200)
