丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Hybrid-Arrested Translation

互联网

610
Hybrid arrested translation (1 ) is a means of identifying recombinant DNA clones by their ability to hybridize to, and thus prevent the translation of, a specific messenger RNA in a cell-free system. In this respect the approach is similar to “hybrid selection,” also known as “hybrid release” (see Chapter 3 ), in which messenger RNAs homologous to cDNA clones are specifically trapped as RNA/DNA hybrids on nitrocelloulose filters. By washing the filters at specific temperatures and salt concentrations, RNA molecules bound nonspecifically are removed leaving only the RNA homologous to the DNA bound. This RNA can then be released by boiling the filter, precipitated with ethanol, and translated in vitro. The fundamental difference between the two approaches is that, whereas in hybrid selection translation reveals one or a few polypeptides, hybrid-arrested translation identifies a gene product by its disappearance from the pattern of translation products.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序