General details of cell culturing
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实验试剂
1. 450 ml DMEM - Remove 50 ml from 500 ml bottle then add the other constituents. |
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实验步骤
1. Preparing an aseptic environment
2) Autoclaving
a. Pipette tips (or can be purchases pre-autoclaved, DNAse/RNAse free)
3) b. Glass 9” Pasteur pipettes
4) c. 70% ethanol (Be sure to spray all surface areas)
2. Preparation of cell growth medium
3. Creating the correct culturing environment
The following is an example for endothelial and epithelial cells:
3) Completely remove coating solution by aspirating before seeding.
They are in quiesence (do not appear to be growing at all).
注意事项