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Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages

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Caspase-1 is a critical factor in the innate immune response to Legionella pneumophila . The development of methods for analyzing caspase-1 activation pathways and downstream caspase-1-associated activities has helped in understanding the regulation of this protease and the signaling components involved. Here we outline methods for directly detecting active caspase-1, measuring caspase-1 activities and analyzing components involved in the regulation of caspase-1 during L. pneumophila infection in macrophages.
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