Preparation of Unnatural Amino Acids with Ammonia-Lyases and 2,3-Aminomutases
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Ammonia-lyases catalyze a wide range of processes leading to α,β-unsaturated compounds by elimination of ammonia. In this chapter, ammonia-lyases are reviewed with major emphasis on their synthetic applications in stereoselective preparation of unnatural amino acids. Besides the synthesis of various unnatural α-amino acids with the aid of phenylalanine ammonia-lyases (PALs) utilizing the 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) prosthetic groups, the biotransformations leading to various unnatural β-amino acids with phenylalanine 2,3-aminomutases using the same catalytic MIO prosthetic group are discussed. Cloning, production, purification, and biotransformation protocols for PAL are described in detail.
![CPS1/CPS1蛋白/Carbamoyl-phosphate synthetase I (CPSase I)蛋白/Recombinant Human Carbamoyl-phosphate synthase [ammonia], mitochondrial (CPS1), partial重组蛋白](https://img1.dxycdn.com/p/s14/2024/0807/669/8231713251822901281.jpg!wh200)
