Propagation of Wild-Type and Mutant SV40
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Simian virus 40 (SV40) is routinely propagated in established kidney cell lines derived from the African green monkey (1 ). Generally, one of two cell lines, BSC-1 or CV1, are used for this purpose (2 ,3 ). Both of these cell lines are easy to maintain in culture and can be readily frozen and recovered from frozen storage. Wild-type SV40 grows and plaques equally well in both cell lines.
