ACID PICRO MALLORY

互联网2013-09-06

1076

1. Bring sections to water

2. Stain in Celestine blue, 3 mins

3. Wash in water

4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water

6. Blue in Scotts tap water

7. Wash in water

8. Rinse in 95% alcohol

9. Stain in Picro-orange 2 mins

10. Wash in water until only red blood cells are yellow

11. Stain in acid fuchsin 5 mins

12. Wash in water

13. Stain in equal parts Picro-orange and 80% alcohol

14. Differentiate in phosphomolybdic acid for 8 mins

15. Wash in water

16. Stain in analine blue, 8 mins

17. Wash in water

18. Dehydrate, clear . Mount sections in DPX

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