丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Measurement of Vascular Density

互联网

427
Determination of the number of microvessels in tissue is of great importance in the assessment of studies of vascular development, angiogenesis, and rarefaction (1 ). Numerous techniques, such as immunohistochemical staining, fluorescence injection, and India ink or microfil perfusion have been developed for microvascular visualization. In this section, we describe a micro-vascular visualization technique (2 ), which utilizes rhodamine-labeled Griffonia simplicifolia (GS-I) lectin to define both perfused and unperfused microvessels ranging from small arteries (20 μm) to normal capillaries (3-6 μm) to venous capillaries (6-9 μm), combined with a computerized image processing technique that rapidly and automatically determines vascular density in tissue sections (3 ).
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序