Use of Monoclonal Antibodies in Immunoelectron Microscopy

Immunoelectron microscopy (IEM) techniques, usually in conjunction with light microscopy techniques, are used for the localization of antigenic molecules that may be employed for vaccine development, organelle characterization, or other biologically relevant applications. Typically, the steps involved in IEM include fixation, dehydration and embedding, cutting and mounting, immunolabeling, observation, and documentation.