Immunoelectron Microscopy

The immunolocalization of proteins on sections of biological materials that have been prepared for electron microscopy, requires the preservation of both antigenic and morphological structures. In this chapter, two techniques that fulfill these requirements, often difficult to reconcile, but that have been used extensively for mammalian cells, will be described in their adaptation for yeast cells: the progressive low temperature embedding technique in Lowicryl introduced by Carlemalm et al. (1 ) and cryo-ultramicrotomy as described by Tokuyasu (2 ). The immunolocalization technique used in this chapter is based on the protein-A gold technique described by Roth et al. (3 ) and Geuze et al. (23 ).