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Development of Simian Retroviral Vectors for Gene Delivery

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The development of retroviral gene transfer vectors has provided an effective way to deliver foreign genes into mammalian cells. This chapter will focus on the development of a simian retrovirus (SRV) gene delivery system. This includes the identification of the minimal packaging signal of a type D serogroup 2 SRV D2/RHE/OR/V1, construction of the SRV transduction and packaging vectors, generation of the SRV specific packaging cell line and production of the replication-deficient recombinant SRV.
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