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Construction of Adenoviral Vectors

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Human adenovirus is a double-stranded DNA virus with a 36-kb linear genome that contains four early transcription units (E1–E4), active at early (3–12 h) times after infection and five late transcription units (L1–L5) active thereafter and producing the structural genes of the virus. For further details on human adenovirus and its life cycle, the reader is referred to ref. 1 , which provides a description of the genome structure, and types of these viruses.
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