Generation of Transgenic Animals with Lentiviral Vectors

In this chapter, we will discuss the use of lentiviral vectors to generate transgenic animals. Specifically, we will review the technology as applied to the generation of rodents and birds. But many of the procedures can be applied in other species. We review the production of vectors, their design and construction, promoters and reporters. We also discuss techniques for the introduction of vectors to rodent zygotes and bird eggs.