The Technique of In Situ Hybridization: Principles and Applications

The aim of this chapter is to give a general overview of the technique of in situ hybridization By way of introducing the technique, the history and background of in situ hybridization will first be discussed, followed by the principles and basic steps involved and lastly, the various applications of in situ hybridization Detailed descriptions of the individual procedures and methods are outside the scope of this chapter, but readers are referred to In Situ Hybridization Protocols , edited by K H. Andy Choo, 1994, and Nonradioactive In Situ Hybridization Application Manual , Boehnnger Mannheim, 2nd Edition, 1996.