Antibody Select Procedure for Characterization of Expression Clones

This chapter essentially combines a variety of techniques already described in other chapters to allow a rapid characterization or con firmation of λ expression clones. Antibodies used to screen expres sion libraries (see Chapter 21 ) may have been raised against affinity purified antigen (see Chapter 29 ), which can never be guaranteed free of contaminating material, or may even have been produced by an infected host during the course of a parasitic infection. Obviously such sera may recognize more than one antigen, and it is essential for the char acterization of the gene that has been cloned using such sera that the antibodies and gene product generating the positive signal can be identified.