丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Small-Scale Plasmid DNA Preparation

互联网

567
For the initial characterization of a recombinant plasmid, it is necessary to determine the size of the plasmid or, preferably, the size and characteristics of the insert itself. A method is therefore required for the simultaneous preparation, from a number of isolates, of plasmid DNA in a state sufficiently pure for restriction enzyme digestion. The requirements of such a procedure are:
(i) 
A simple method for rapid lysis of the bacterial cells.
(ii) 
Separation of plasmid from chromosomal DNA.
(iii) 
Removal of proteins and of other components of the cells that might interfere with restriction enzyme treatment.
(iv) 
Removal of detergents, salts, etc. used in the process.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序