Amicon Stirred Ultrafiltration Cells
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Amicon Stirred Ultrafiltration Cells
(Models 8050, 8400)
Description - Membranes - Assembly - Operation - Limits - Sterilization - Maintenance - Troubleshooting
Description
For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.Membranes
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YM10 Ø43 mm (for 8050), Amicon #13622, 10 pack: $108
YM10 Ø76 mm (for 8400), Amicon #13642, 10 pack: $139 - Prewash: float membrane with glossy side down in beaker with distilled water for at least 1 hour changing water 3 times . All membranes are pretreated with glycerine to prevent drying, YM membranes are treated with sodium azide as a preservative.
- Cleaning: rinse with 0.1M NaOH, followed by thorough flushing with distilled water. For strongly absorbed protein, soak in 0.1% protease solution and rinse thoroughly.
- Storage: for reuse, store membranes in 10% ethanol water solution at 4°C.
Assembly
- Place membrane in holder, shiny side up ; then place O-ring on top of membrane. Gently push O-ring down so that it contacts and seats membrane evenly in bottom of holder. Handle membrane by its edges to avoid scratching or contaminating surface.
- Fit membrane holder into cell body, aligning tabs on sides of holder with slots in base of cell body.
- Invert cell body and membrane holder; screw base firmly into bottom of cell body. A definite "stop" will be felt when base and body are fully engaged. Top of membrane holder will be flush with bottom of slots in cell body.
- Push filtrate exit tubing onto exit spout of membrane support.
- Place stirrer assembly into cell body. When properly installed, arms of stirrer assembly will rest on small ridge inside top of cell body.
- Introduce sample into cell.
- With a twisting motion, push cap down onto cell body, orienting gas inlet port on cap opposite filtrate exi
