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[欢迎下载]:Antibody Engineering: Methods and Protocols

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内容:Antibody Engineering: Methods and Protocols
格式:pdf
大小:5.18M
语种:英文
简介:为Methods in Molecular Biology系列第248卷,由Benny K. C. Lo主编,Humana Press 2003年出版。目录如下:
1 Internet Resources for the Antibody Engineer
2 The Kabat Database and a Bioinformatics Example
3 IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr
4 Antibody Variable Regions: Toward a Unified Modeling Method
5 Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
6 PCR Cloning of Human Immunoglobulin Genes
7 Antibody Humanization by CDR Grafting
8 Selection of Human Antibodies from Phage Display Libraries
9 Production of Human Single-Chain Antibodies by Ribosome Display
10 Production of Human Antibodies from Transgenic Mice
11 Selection of Internalizing Antibodies for Drug Delivery
12 Engineering Multivalent Antibody Fragments for In Vivo Targeting
13 Production of Recombinant Bispecific Antibodies
14 Expression and Isolation of Recombinant Antibody Fragments in E. coli
15 Expression of Recombinant Antibodies in Mammalian Cell Lines
16 Human Antibody Production Using Insect-Cell Expression Systems
17 Antibody Production in Transgenic Plants
18 Directed Mutagenesis of Antibody Variable Domains
19 Antibody Affinity Maturation by Chain Shuffling
20 Antibody Affinity Maturation by Random Mutagenesis
21 Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
22 Antibody Purification by Column Chromatography
23 Affinity Measurement Using Surface Plasmon Resonance
24 Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions
25 Characterization of Antibody–Antigen Interactions by Fluorescence Spectroscopy
26 Antibody Epitope Mapping Using Arrays of Synthetic Peptides
27 Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
28 Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
29 Production and Characterization of Anti-Cocaine Catalytic Antibodies
30 Recombinant Immunotoxins in the Treatment of Cancer
31 Antibodies in Proteomics
32 Targeting of Antibodies Using Aptamers
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