Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition

互联网2014-02-13

371

A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM™). SCAM™ is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.

相关产品推荐

GST pull-down试剂盒|GST 蛋白相互作用 Pull-Down 试剂盒|高效纯化/灵敏度高/特异性强| GST pull-down kit| GST Protein Interaction Pull-Down Kit

￥379

Klrg1/Klrg1蛋白/(Mast cell function-associated antigen 2F1)蛋白/Recombinant Mouse Killer cell lectin-like receptor subfamily G member 1 (Klrg1), partial重组蛋白

￥69

赛默飞世尔Thermo Fisher PUMP APPARATUS STUDY GUIDE 7TH 货号：T_70119-819-195

￥1200

F13L/F13L蛋白/(37 kDa protein)(Envelope protein F13)(Palmitoylated EV membrane protein)(p37K)蛋白/Recombinant Vaccinia virus Envelope phospholipase F13 (F13L)重组蛋白

￥69

LTP2/LTP2蛋白/Allergen Par j II Major pollen allergen Par j 2.0101 Protein P2 Allergen: Par j 2.0101蛋白/Recombinant Parietaria judaica Probable non-specific lipid-transfer protein 2 (LTP2)重组蛋白

￥69