Zebrafish Immunohistochemistry

Immunohistochemistry is a powerful technique for determining both the presence of and the subcellular location of proteins within tissues. Zebrafish are particularly amenable to this technique and it is possible to localize proteins both in whole embryos and larvae, as well as sectioned material (Fig. 1 ). In this chapter, I will describe the basic technique developed for wholemount labeling of zebrafish embryos and variations that are required in specific situations. In addition, for each technique, pitfalls will be highlighted and ways to avoid these suggested.