基因敲除--Gene Knockout Protocols
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基因敲除--Gene Knockout Protocols
Methods in Molecular Biology VOLUME158
Gene Knockout Protocols 基因敲除实验方法
M. J. Tymms and I. Kola
Humana Press Inc.
PDF格式，共437页，4.5M，点击此处下载
内容：
1 Overview Gene Knockouts
2 Isolation and Maintenance of Primate Embryonic Stem Cells
3 Gene Targeting in ES Cells
4 Manipulating Mouse Embryonic Stem Cells
5 Gene Targeting in a Centralized Facility
6 The LoxP/CRE System and Genome Modification
7 Creation and Use of a Cre Recombinase Transgenic Database
8 Choice of Mouse Strains for Gene Targeting
9 Isolation, Microinjection, and Transfer of Mouse Blastocysts
10 Aggregation Chimeras
11 How to Study Pathologic Phenotypes of Knockout Mice
12 Analysis of Hematopoietic Phenotypes in Knockout Mouse Models
13 Isolation of Embryonic Fibroblasts and Their Use in the In Vitro
Characterization of Gene Function
14 Influence of Genetic Background on Knockout Mouse Phenotypes
15 Lineage Allocation During Early Embryogenesis
16 Generation of Double-Knockout Embryonic Stem Cells
17 In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes
18 Embryonic Stem Cells in the Study of Hematopoiesis
19 Interferon-Inducible ES Cell Expression Systems
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