Automated Three‐Chambered Social Approach Task for Mice
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- Abstract
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Abstract
Autism is diagnosed by three major symptom categories: unusual reciprocal social interactions, impaired communication, and repetitive behaviors with restricted interests. Direct social approach in mice has strong face validity to simple social approach behaviors in humans, which are frequently impaired in autism. This unit presents a basic protocol for a standardized, high?throughput social approach test for assaying mouse sociability. Our automated three?chambered social approach task quantifies direct social approach behaviors when a subject mouse is presented with the choice of spending time with either a novel mouse or a novel object. Sociability is defined as the subject mouse spending more time in the chamber containing the novel target mouse than in the chamber containing the inanimate novel object. The Basic Protocol describes procedures for testing one subject at a time in a single apparatus. A Support Protocol addresses data collection. Curr. Protoc. Neurosci. 56:8.26.1?8.26.16. © 2011 by John Wiley & Sons, Inc.
Keywords: autism; social behaviors; sociability; social approach; three?chambered task; mouse models; automated behavioral test
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PDF or HTML at Wiley Online LibraryTable of Contents
- Introduction
- Basic Protocol 1: Automated Three‐Chambered Social Approach Task
- Support Protocol 1: Data Collection
- Commentary
- Literature Cited
- Figures
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Basic Protocol 1: Automated Three‐Chambered Social Approach Task
Materials
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Figure 8.26.1 The social approach apparatus is a rectangular three‐chambered box. Each chamber measures 20 cm (length) × 40.5 cm (width) × 22 cm (height). Dividing walls are made from clear Plexiglas, with small openings (10‐cm width × 5‐cm height) that allow access into each chamber. The center chamber is the start location. The weighted cup on top of the wire cup prevents climbing. The empty wire cup in the right chamber is the novel object. Photobeams are embedded across each doorway. An automated photobeam detector registers time spent in each chamber and number of transitions. This social approach apparatus and the accompanying software program are designed and manufactured by Dold Labs and Engineering. View Image -
Figure 8.26.2 Stopwatches that emit a beeping sound can be silenced by removing an internal metal part from the inner surface of the back panel. View Image -
Figure 8.26.3 Adult male C57BL/6J (B6) and FVB/AntJ mice displayed sociability, defined as spending more time in the chamber containing the novel mouse than in the chamber containing the novel object (A ), and more time sniffing the novel mouse than sniffing the novel object (B ). Adult male BTBR T+ tf /J (BTBR) mice did not display sociability, spending similar amounts of time in the chamber containing the novel mouse and in the chamber containing the novel object (A), and similar amounts of time sniffing the novel mouse and sniffing the novel object (B). B6 ( n = 12), FVB/Ant ( n = 16) and BTBR ( n = 12). The asterisks ( View Image -
Figure 8.26.4 Significant correlation between time spent in chamber and sniff time in C57BL/6J adult male mice ( n = 72; M. Yang, M.D. Weber, A.M. Clarke, and V. Zhodzishsky, unpub. observ.). View Image
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