Quick Yeast DNA Prep: Isolation of Total DNA (genomic and plasmid)
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Lysis buffer: | 20 ml: | |
0.1M Tris pH 8.0 | 2 ml 1M Tris 8.0 | |
50 mM EDTA | 2 ml 0.5M EDTA | |
1% SDS | 2 ml 10% SDS | |
14 ml H2 O | ||
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Lysis buffer: | 20 ml: | |
0.1M Tris pH 8.0 | 2 ml 1M Tris 8.0 | |
50 mM EDTA | 2 ml 0.5M EDTA | |
1% SDS | 2 ml 10% SDS | |
14 ml H2 O | ||
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