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Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells

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There has been great interest in ex vivo expansion of human long-term repopulating hematopoietic stem cells (LTR-HSC) for a variety of developing clinical applications including HSC transplantation, gene therapy, and production of mature blood cells in manufacturing. Because the development of HSC is thought to be regulated, at least in part, by interactions of cytokine receptor signals, many investigators used various combinations of cytokines that have been shown to act on primitive hematopoietic cells to obtain the optimal culture condition for HSC expansion.
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