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Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes

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Despite a 10,000-fold gradient of Ca2+ across the cell membrane, the concentration of cytosolic free calcium, [Ca2+ ]i , is regulated with remarkable constancy. A combination of mechanisms precisely regulate [Ca2+ ]i at nanomolar levels. These include influx of Ca2+ via plasma membrane calcium channels, release and redistribution of Ca2+ from internal stores, and efflux of Ca2+ by the action of ATP-driven calcium pumps.
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