Lipidomics: Extraction Protocols for Biological Matrices

Lipidomics is defined as a comprehensive analysis of all lipids in a biological system (lipidome). Lipid profiling is a fast-growing area of research due to the involvements of lipids incorporated with human diseases such as obesity, Alzheimer’s diseases, and diabetes as well as in drug discovery. Recently improved liquid chromatography and mass spectrometric techniques has provided the rapid expansion of lipidomics research. Lipid extraction and sample preparation is an important step in lipidomics and this chapter describes lipid extractions from different types of samples such as plasma, tissues, yeast, and cells.