Challenges of Sticky Co-immunoprecipitation: Polyalanine Tract ProteinProtein Interactions
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Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein
interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties
using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult
PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
