Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay
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Sperm selection is an important part of the ICSI procedure. Routinely, sperm selection for ICSI is based solely on sperm morphology and motility. These latter parameters may not be sufficient to select sperm with intact chromatin. Therefore, sperm selected based on sperm functional characteristics may result in the most appropriate sperm for the ICSI procedure. The methodology explained below describes the selection of sperm based on the ability of sperm to bind solid-state hyaluronic acid as its receptor, present on mature sperm with intact chromatin.
