Label-Free Detection with the Resonant Mirror Biosensor
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The resonant mirror (RM) biosensor is a leaky waveguide-based instrument that uses the evanescent field to probe changes in
the refractive index at the sensing surface.The RM can therefore be used to monitor in real-time and label-free the interaction
between an analyte in solution and its biospecific partner immobilized on the waveguide surface.The RM has been used in studying
the interaction of a variety of moieties including proteins, carbohydrates, cells, nucleic acids and receptors, leading to
applications in areas such as clinical diagnostics, homeland security, and pharmaceutical and biomolecular interactions. This
chapter will review the principle of this biosensor, and the recent advances in instrumentation, different immobilization
chemistries, and kinetic studies, as well as some applications.