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Hepatic Portal Branch Inoculation

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The study of plasmodial liver stage parasites, in vivo, has been hampered by the relatively small number of liver stage schizonts that can be generated in an animal. This has been true even when large numbers of sporozoites are injected. The number of liver stage parasites can be increased, by increasing the parasite inoculum, but the liver stage schizonts plateau at a sporozoite dose of 3-6�106 (1 ). It is not clear why this occurs, but the result is that the number of infected hepatocytes is small, relative to the noninfected hepatocytes.
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