Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Antibody–antigen interactions can principally be classified into three different temperature-dependent kinetic rate profiles. The affinity K _D can persist, decrease, or increase in the temperature gradient. Today, the impact of temperature-dependent antibody kinetics is recognized, especially as part of the development of best in class monoclonal antibodies. Here, a robust surface plasmon resonance-based protocol is presented, which describes a sensitive temperature-dependent kinetic measurement and evaluation method.