Preparation of Ovine Adenovirus Vectors

Human adenovirus vectors have been used widely as gene delivery vehicles. However, one major problem related to the use of these vectors is the presence of a preexisting immunity to human adenoviruses in a majority of the population. We (1 –3 ) and others (4 –6 ) are therefore developing nonhuman adenoviruses as gene therapy vectors. Among these, vectors derived from the ovine adenovirus isolate 287 (OAV) have been found to deliver genes with high efficiency to several mammals. This chapter introduces this novel vector system and describes the methodology of generating OAV vectors.