Detection of Anti-Sense RNA Transcripts by Anti-Sense RT-PCR

Reverse-transcriptase-polymerase chain reaction (RT-PCR) is a powerful and sensitive tool for RNA detection. This technique is particularly valuable in cases where the amount of an RNA species present is so minute that traditional RNA analysis methodologies such as Northern blot or RNase protection assay may not be adequate. It has been estimated that RT-PCR is thousands of times more sensitive than Northern blot analysis (1 )