Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
互联网
433
In this article we provide a method to isolate hair follicle stem cells that have undergone targeted gene inactivation. The
mice from which these cells are isolated are bred into a Rosa26-
yellow fluorescent protein (YFP) reporter background, which results in YFP expression in the targeted stem cell population.
These cells are isolated and purified by fluorescence-activated cell sorting, using epidermal stem cell-specific markers in
conjunction with YFP fluorescence. The purified cells can be used for gene expression studies, clonogenic experiments, and
biological assays, such as viability and capacity for directional migration.